Inhibition of γ/β Globin Gene Switching in CD 34+ Derived Erythroid Cells by BCL11A RNA Silencing
نویسندگان
چکیده
منابع مشابه
MicroRNA-486-3p Regulates γ-Globin Expression in Human Erythroid Cells by Directly Modulating BCL11A
MicroRNAs (miRNAs) play key roles in modulating a variety of cellular processes through repression of mRNAs target. The functional relevance of microRNAs has been proven in normal and malignant hematopoiesis. While analyzing miRNAs expression profile in unilineage serum-free liquid suspension unilineage cultures of peripheral blood CD34(+) hematopoietic progenitor cells (HPCs) through the eryth...
متن کاملPromoter Methylation and Gene Expression in Human CD34+ Stem Cells Derived Erythroid Lineage by MicroRNA
Background: Stem Cell differentiation is a process composed of vast variety of factors which are controlled by a network of certain mechanisms. This study aims to determine the possible role of DNA methylation, a potent regulator of VHL, ECAD and RUNX3 genes during Erythroid differentiation driven by miR-451. Materials and Methods: To determine the methylation status of promoters and the e...
متن کاملErythropoiesis and globin switching in compound Klf1::Bcl11a mutant mice.
B-cell lymphoma 11A (BCL11A) downregulation in human primary adult erythroid progenitors results in elevated expression of fetal γ-globin. Recent reports showed that BCL11A expression is activated by KLF1, leading to γ-globin repression. To study regulation of erythropoiesis and globin expression by KLF1 and BCL11A in an in vivo model, we used mice carrying a human β-globin locus transgene with...
متن کاملThe role of the polycomb complex in silencing α-globin gene expression in non-erythroid cells
Although much is known about globin gene activation in erythroid cells, relatively little is known about how these genes are silenced in non-erythroid tissues. Here we show that the human αand β-gobin genes are silenced by fundamentally different mechanisms. The α-genes, which are surrounded by widely expressed genes in a gene dense region of the genome, are silenced very early in development v...
متن کامل"Maturational" globin switching in primary primitive erythroid cells.
Mammals have 2 distinct erythroid lineages. The primitive erythroid lineage originates in the yolk sac and generates a cohort of large erythroblasts that terminally differentiate in the bloodstream. The definitive erythroid lineage generates smaller enucleated erythrocytes that become the predominant cell in fetal and postnatal circulation. These lineages also have distinct globin expression pa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Indian Journal of Hematology and Blood Transfusion
سال: 2019
ISSN: 0971-4502,0974-0449
DOI: 10.1007/s12288-019-01131-8